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Ten-man Paraguay beat Turkey 1-0 at the World Cup on Friday, a result that kept their hopes alive and also guaranteed that co-hosts the USA win Group D.
Turkey's defeat means they become the second team after Haiti to be eliminated from contention.
Matias Galarza scored the only goal in a game that featured the first sending off in World Cup history for a player covering their mouth during an on-field confrontation, for Miguel Almiron.
Galarza's low, long-range shot evaded Turkey goalkeeper Ugurcan Cakir after just 63 seconds.
Former Newcastle forward Almiron was given his marching orders in first-half stoppage time after covering his mouth while he appeared to direct a comment at Turkey's Mert Muldur.
In a scrappy match, Deniz Gul had a golden chance to equalize in the 88th minute when Paraguay 'keeper Orlando Gill could only parry a shot, but he stabbed his shot wide of the goal.
Merih Demiral glanced a header wide in the dying seconds and was almost in tears afterwards as the Turkey players slumped to the turf in bitter disappointment.