Kibwezi West MP Patrick Musimba and his wife Angela Mwende have been charged with conspiracy to defraud Chase bank of Sh1 billion.
The two denied the charges before Milimani Senior Resident Magistrate Hellen Onkwani and were remanded at the Kileleshwa Police Station until September 20 pending a bail hearing.
State counsel Mungai Warui opposed bond for the couple and four others.
Musimba and his wife were charged alongside the bank's former former chairman Mohammed Khan and several senior managers among them Dancun Kabui, James Mwenja, Makrios Agumbi, Luciane Sunter and Ronald Vlasman.
On Monday the court released Mr Khan on a Sh2m cash bail and allowed to travel to the US for medical treatment.
The court had issued an arrest warrant for both on Friday last week after they failed to appear on Thursday.
According to the charge sheet, Musimba and co-accused conspired to defraud the bank by irregularly disbursing the money to Porting Access Limited and Itecs Limited between January 23, 2015 and March 31, 2016.
It says this was done in disregard of the bank's payment process.