On several occasions I have been involved in cases of vaccine failure in poultry - either due to poor administration techniques of the vaccine or due to break of the “cold chain”. Cold chain refers to the temperature-controlled supply chain under which a vaccine should be transported and stored from the time of production until it gets to be administered in the farm.
The vaccine should be refrigerated at temperatures between three and eight degrees celcius. If this is not observed then the quality and potency of the vaccine is tampered with and possibilities of vaccine failure are high.