Aston Villa captain Jack Grealish has received his first call-up to the senior England men’s team.
Southgate was criticised when he left out Grealish in his first announcement on August 25.
Grealish was part of Southgate's under-21 2016 squad that was successful in Toulon.
The England boss also gave first call-ups to Wolverhampton Wanderers defender Conor Coady and Arsenal left-back, Ainsley-Maitland Niles.
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Man United striker Marcus Rashford and Tottenham’s Harry Winks had to withdraw.
Gutted ???? never want to let this team down but these fixtures just came a little too soon for me. I tried my best but I have to focus on starting the season at my strongest for club and country. Good luck boys, I’ll be cheering you on from home ????♥? @England pic.twitter.com/2OMHKPFLpm — Marcus Rashford (@MarcusRashford) August 31, 2020
Tammy Abraham (Chelsea), Trent Alexander-Arnold (Liverpool), Conor Coady (Wolverhampton Wanderers), Eric Dier (Tottenham Hotspur), Phil Foden (Manchester City), Joe Gomez (Liverpool), Jack Grealish (Aston Villa), Mason Greenwood (Manchester United), Dean Henderson (Manchester United), Danny Ings (Southampton), Harry Kane (Tottenham Hotspur), Michael Keane (Everton), Ainsley Maitland-Niles (Arsenal), Tyrone Mings (Aston Villa), Mason Mount (Chelsea), Kalvin Phillips (Leeds United), Jordan Pickford (Everton), Nick Pope (Burnley), Declan Rice (West Ham United), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), Kieran Trippier (Atletico Madrid), Kyle Walker (Manchester City), James Ward-Prowse (Southampton)
COURTESY: ENGLAND WEBSITE (FA)
Manchester United defender Harry Maguire was initially named in the squad but had to be withdrawn following his incident in Mykonos, Greece.
Maguire was detained after allegedly being involved in a brawl in which police officers were reportedly assaulted.
He faced charges by a public prosecutor and received a suspended prison sentence of 21 months, 10 days. Maguire says he is innocent and has been allowed full retrial after appealing against the verdict.
England play Iceland in Reykjavik on Saturday, September 5 then travel to Copenhagen on Tuesday, September 8 to face Denmark.