Mr Poots said he had received two new pieces of research this week which have strengthened his position.
Speaking on the Sunday Politics programme he said he did not want the ban to apply to gay people only.
"I think that people who engage in high risk sexual behaviour in general should be excluded from giving blood," the minister said.
"And so someone who has sex with somebody in Africa or sex with prostitutes, I am very reluctant about those people being able to give blood."
Mr Poots said a ban on blood donations from gay people should also apply to people who have sex "with somebody in Africa or sex with prostitutes".
He responded to the criticism on Tuesday, saying there "have been measures in place for decades that prohibit blood being given by people who have had sex in Africa, with a 12 month deferral".
"This is not something new," he said.
"In many African countries we have high levels of HIV and therefore it is incumbent on us to protect the safety of individuals receiving blood."
Donald Makony from ACSONI had told the BBC World Service Focus on Africa programme that people in his community were "outraged" that someone at such a senior level in government was expressing such "backdated" views.
He said he was not really surprised by the remarks but added: "It was just more disgust that the man can just say these things and not really expect any kind of reaction from the people he's talking about in such a derogatory way."