A close friend said Wilson - who also appeared in the TV series The Parkers and the movies House Party 2 and 3 - died of cervical cancer.
Wilson's cancer returned recently after several years in remission, said Jeffrey Pittle.
Pittle made the announcement on a website he had set up to raise funds for Wilson's treatment.
"It is with a heavy heart that I can verify that Yvette passed away last evening after a lengthy and hard battle," he said.
"She was a fighter to the end, and her talent, humour and amazing friendship will be sorely missed. She will live on through her awesome body of work."
Wilson portrayed Andell Wilkerson, owner of the popular hangout ‘The Den on Moesha.’
Wilson's Moesha co-star Shar Jackson said on Twitter: "Oh god...My heart is soooo unbelievably broken," adding later: "I wanna thank all my tweeties for their prayers but god has chosen to take my sister Yvette home...".
Ray actor Jamie Foxx tweeted: "God bless u Yvette Wilson. Tears in my eyes. Keep God laughing."
Fugees singer Lauryn Hill wrote her on website: "R.I.P Yvette Wilson (Andell From The Parkers). She recently lost her battle with cancer. Life is short, so cherish yours."